Hlavní serologickou metodou používanou v naší laboratoři je ELISA (enzyme-linked immunosorbent assay). K ověření výsledků ELISA testů je používána IFA (immunofluorescence assay), která slouží také jako hlavní metoda k detekci protilátek proti některým nežádoucím mikroorganismům (např. Pneumocystis sp., Chlamydophila psittaci,...). Další metody používané v serologické diagnostice jsou: MAT (micro-agglutination test) sloužící k detekci protilátek proti bakteriím rodu Leptospira, RPR test (rapid plasma reagens) používaný k detekci protilátek proti Treponema paraluiscuniculi.
Viry
| Agens/Zkratka | Metoda | Hostitel |
|---|---|---|
| Sendai virus/Murine parainfluenza virus 1 (SEND/MPIV-1) | ELISA, IFA | M, R, H, Gp, Rb |
| Parainfluenza virus 5/Simian virus 5 (PIV-5/SV5) | ELISA, IFA | H, Gp, Rb |
| Pneumonia virus of mice/Murine pneumonia virus (PVM) | ELISA, IFA | M, R, H, Gp, Rb |
| Parainfluenza virus 3 (PIV-3) | ELISA, IFA | Gp |
| Mouse hepatitis virus (MHV) | ELISA, IFA | M |
| Sialodacryoadenitis virus/Rat coronavirus (SDAV/RCV) | ELISA/IFA | R |
| Mouse parvovirus (MPV) | ELISA, IFA | M |
| Minute virus of mice (MVM) | ELISA, IFA | M |
| Kilham rat virus (KRV) | ELISA, IFA | R |
| Rat parvovirus (RPV) | ELISA, IFA | R |
| Toolan's H-1 virus (H-1) | ELISA, IFA | R |
| Rat minute virus (RMV) | ELISA | R |
| Non-structural parvoviral protein (NS-1) | ELISA | M, R |
| Theiler's murine encephalomyelitis virus (TMEV) | ELISA, IFA | M |
| Rat theilovirus (RTV) | ELISA, IFA | R |
| Mouse rotavirus (EDIM) | ELISA, IFA | M |
| Rabbit rotavirus (ROTA) | ELISA, IFA | Rb |
| Reovirus type 3 (REO-3) | ELISA, IFA | M, R, H, Gp, Rb |
| Ectromelia virus (ECTRO) | ELISA, IFA | M |
| Myxoma virus (POX) | ELISA, IFA | Rb |
| Lymphocytic choriomeningitis virus (LCMV) | ELISA, IFA | M, R, H, Gp, Rb |
| Mouse adenovirus (MAD 1+2) | ELISA, IFA | M, R, H |
| Guinea pig adenovirus (GPAdV) | IFA | Gp |
| Mouse pneumonitis virus (K) | ELISA, IFA | M, R, H |
| Polyomavirus (POLY) | ELISA, IFA | M, R, H |
| Murine norovirus (MNV) | ELISA, IFA | M |
| Rabbit hemorrhagic disease virus (RHDV) | ELISA | Rb |
| Mouse thymic virus (MTV) | IFA | M |
| Mouse cytomegalovirus (MCMV) | ELISA, IFA | M |
| Guinea pig cytomegalovirus (GpCMV) | ELISA, IFA | Gp |
| Hantaan virus (HANT) | ELISA, IFA | M, R |
| Prospect Hill virus (PHV) | IFA | M |
| Lactate dehydrogenase elevating virus (LDHV) | ELISA | M |
Bakterie
| Agens/Zkratka | Metoda | Hostitel |
|---|---|---|
| Mycoplasma pulmonis (MPUL) | ELISA, IFA | M, R |
| Filobacterium rodentium/Cilia-associated respiratory bacillus (CARB) | ELISA, IFA | M, R, Rb |
| Clostridium piliforme (CPIL) | ELISA, IFA | M, R, H, Gp, Rb |
| Chlamydophila psittaci (CHLAM) | IFA | Gp |
| Leptospira spp. (LEPTO) | MAT | M, R, H, Gp, Rb |
| Treponema paraluiscuniculi (TREP) | RPR | Rb |
Paraziti, mikrosporidie a pneumocysty
| Agens/Zkratka | Metoda | Hostitel |
|---|---|---|
| Toxoplasma gondii (TOXO) | IFA | M, R, H, Gp, Rb |
| Encephalitozoon cuniculi (ECUN) | ELISA, IFA | M, R, H, Gp, Rb |
| Pneumocystis carinii (PCAR) | IFA | R |
Použité zkratky: M - myš, R - potkan, Gp - morče, H - křeček, Rb – králík
